Metschnikowia

Metschnikowia pulcherrima colonies (smaller orange colonies) interspersed among Saccharomyces cerevisiae (larger pink colonies), from Morata et al. (2019).

Belongs within: Saccharomycetales.

A barely pronounceable yeast
Published 7 November 2007

“Yeast” is, admittedly, not one of the most tasteful-sounding words in the English language (it’s right up there with “moist” and “sphagnum” in the category of words that sound crude without necessarily meaning anything offensive) but yeasts are an abundant component of our environment. They are most familiar as a vital component in making many foods (such as the baker’s or brewer’s yeast, Saccharomyces cerevisiae, in bread and beer) or less welcomely as opportunistic pathogens (such as Candida albicans, the cause of thrush). Technically speaking, yeasts are fungi that have abandoned the hyphal growth-form of most fungi and reverted to a more unicellular mode of life. Phylogenetically, yeasts are scattered throughout the fungal family tree, and it is this scattered distribution among more standard hyphal fungi that suggests that the yeast form has arisen multiple times independently.

Metschnikowia pulcherrima, from the INRA.

The largest grouping of yeasts is the class Saccharomycetes. While morphologically fairly uniform (lets face it, there’s only so much you can do with a relatively unornamented unicell), Saccharomycetes show a reasonable degree of diversity in biochemistry. The most recent (2006) edition of the Outline of Ascomycota (Eriksson 2006—the kind-of-sort-of official classification of ascomycete fungi) places all Saccharomycetes in a single order, the Saccharomycetales, but I personally prefer the admittedly less popular idea of dividing Saccharomycetes into a number of orders (as was done, for instance, by Schweigkofler et al. 2002) as I feel that this (a) better reflects said chemical diversity, and (b) reduces the redundant uninformativeness of having only a single order in a single class.

Within the Saccharomycetales (however you want to define it), the genus Metschnikowia contains spherical to cylindrical yeasts that reproduce by multilateral budding and produce elongate to club-shaped asci containing one or two needle-shaped spores each (Miller et al. 1967). Successive rounds of budding may lead to the formation of a pseudomycelium. The presence of only two instead of the expected four spores per ascus is due to the spores originating after meiosis stage I (segregation of homologous chromosomes) but before meiosis II (separation of sister chromatids). Meiosis II occurs as per normal after spore division, but one of the resultant nuclei is degraded in each cell (Marinoni et al. 2003). However, I must confess ignorance of what happens in those species with only a single spore per ascus.

Opened ascus of Metschnikowia zobellii showing needle-like spores, from Miller et al. (1967).

Metschnikowia was first discovered as a parasite of water fleas (Daphnia magna, small freshwater crustaceans) but various species and their Candida anamorphs* have since been found in association with a wide range of arthropods and plants (sometimes both – Candida kunwiensis, the anamorph of Metschnikowia kunwiensis – Brysch-Herzberg, 2004 – was isolated in a Korean laboratory from flowers of Ipomoea batatas (kumara) at the same time as German researchers independently isolated it from the bodies of pollinating bumblebees – Hong et al., 2003). Most are probably commensals on their hosts as opposed to parasites.

*An “anamorph” is the asexually reproducing form of a fungus, as opposed to the sexually reproducing teleomorph. Someday I shall write about the complicated and confusing system of double nomenclature attendant on fungi whereby asexual forms are classified separately from sexual forms, and the combination of history, theory and a certain degree of pragmatism that has lead to this system. Today, however, is not that day.

Recently certain Metschnikowia species have gained a bit of attention as potential biocontrol agents, as plant-associated forms have been demonstrated to inhibit the growth of other micro-organisms on their hosts. Metschnikowia yeasts may be very useful in inhibiting the growth of mould on harvested fruit, and at least one patent exists suggesting coating harvested fruit in a mixture containing isolated Metschnikowia culture.

Systematics of Metschnikowia
Metschnikowia Kamieński 1899 (see below for synonymy)KC01
|--+--M. drosophilaeHB03
| `--‘Candida’ torresiiHB03
`--+--+--M. agavesHB03
| |--‘Candida’ intermediaYK99
| |--‘Candida’ lusitaniaeSS09
| `--Clavispora Rodr. Mir. 1979HB03, KC01
| |--C. lusitaniaeHB03
| `--C. opuntiaeHB03
`--+--+--M. pulcherrimaHB03
| `--+--M. lunataHB03
| `--+--M. hibisciHB03
| `--+--M. hawaiiensisHB03
| `--+--M. continentalisHB03
| | |--M. c. var. continentalisHB03
| | `--M. c. var. borealisHB03
| `--+--‘Candida’ ipomoeaeHB03
| `--M. lochheadiiHB03
`--+--+--M. gruessiiHB03
| `--‘Candida’ kunwiensis Hong, Bae et al. 2003HB03
`--+--+--M. koreensisHB03
| `--M. reukaufiiHB03
`--+--M. krissiiHB03
`--+--M. zobelliiHB03
`--+--M. australisHB03
`--M. bicuspidataHB03
|--M. b. var. bicuspidataHB03
|--M. b. var. californicaHB03
`--M. b. var. chathamiaHB03

Metschnikowia Kamieński 1899 [=Monospora Metschn. 1884 non Hochst. 1841, Monosporella Keilin 1920; incl. Anthomyces Grüss 1918 non Dietel 1899, Chlamydozyma Wick. 1964, Metschnikowiella Genkel 1913, Nectaromyces Syd. & Syd. 1919; Metschnikowiaceae]KC01

*Type species of generic name indicated

References

Brysch-Herzberg, M. 2004. Metschnikowia kunwiensis comb. nov., the teleomorph of Candida kunwiensis. FEMS Yeast Research 4: 605–607.

Eriksson, O. E. (ed.) 2006. Outline of Ascomycota—2006. Myconet 12: 1–82.

[HB03] Hong, S. G., K. S. Bae, M. Herzberg, A. Titze & M.-A. Lachance. 2003. Candida kunwiensis sp. nov., a yeast associated with flowers and bumblebees. International Journal of Systematic and Evolutionary Microbiology 53: 367–372.

[KC01] Kirk, P. M., P. F. Cannon, J. C. David & J. A. Stalpers. 2001. Ainsworth & Bisby’s Dictionary of the Fungi 9th ed. CAB International: Wallingford (UK).

Marinoni, G., J. Piskur & M. A. Lachance. 2003. Ascospores of large-spored Metschnikowia species are genuine meiotic products of these yeasts. FEMS Yeast Research 3 (1): 85–90.

Miller, M. W., E. R. Barker & J. I. Pitt. 1967. Ascospore numbers in Metschnikowia. Journal of Bacteriology 94 (1): 258–259.

[SS09] Schoch, C. L., G.-H. Sung, F. López-Giráldez, J. P. Townsend, J. Miadlikowska, V. Hofstetter, B. Robbertse, P. B. Matheny, F. Kauff, Z. Wang, C. Gueidan, R. M. Andrie, K. Trippe, L. M. Ciufetti, A. Wynns, E. Fraker, B. P. Hodkinson, G. Bonito, J. Z. Groenewald, M. Arzanlou, G. S. de Hoog, P. W. Crous, D. Hewitt, D. H. Pfister, K. Peterson, M. Gryzenhout, M. J. Wingfield, A. Aptroot, S.-O. Suh, M. Blackwell, D. M. Hillis, G. W. Griffith, L. A. Castlebury, A. Y. Rossman, H. T. Lumbsch, R. Lücking, B. Büdel, A. Rauhut, P. Diederich, D. Ertz, D. M. Geiser, K. Hosaka, P. Inderbitzin, J. Kohlmeyer, B. Volkmann-Kohlmeyer, L. Mostert, K. O’Donnell, H. Sipman, J. D. Rogers, R. A. Shoemaker, J. Sugiyama, R. C. Summerbell, W. Untereiner, P. R. Johnston, S. Stenroos, A. Zuccaro, P. S. Dyer, P. D. Crittenden, M. S. Cole, K. Hansen, J. M. Trappe, R. Yahr, F. Lutzoni & J. W. Spatafora. 2009. The Ascomycota tree of life: a phylum-wide phylogeny clarifies the origin and evolution of fundamental reproductive and ecological traits. Systematic Biology 58 (2): 224–239.

Schweigkofler, W., K. Lopandic, O. Molnár & H. Prillinger. 2002. Analysis of phylogenetic relationships among Ascomycota with yeast phases using ribosomal DNA sequences and cell wall sugars. Organisms, Diversity and Evolution 2: 1–17.

[YK99] Yamada, Y., H. Kawasaki, Y. Nagatsuka, K. Mikata & T. Seki. 1999. The phylogeny of the cactophilic yeasts based on the 18S ribosomal RNA gene sequences: the proposals of Phaffomyces antillensis and Starmera caribaea, new combinations. Bioscience, Biotechnology, and Biochemistry 63: 827–832.

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